The taxa determination in this present study is based on regular systematic survey of more than 300 sampling sites (number and localization of sites were updated annualy). Presence of Philopotamidae representatives: Philopotamus montanus (Donovan, 1813), Philopotamus ludificatus McLachlan, 1878 and Odontoceridae: Odontocerum albicorne (Scopoli, 1763) was recorded in other river basins in comparison with those in the catalogue of Šporka (ed.) 2003.

New records of Ph. ludificatus and O. albicorne were examined in four and three river basins respectively (Tab. 1). Ph. montanus occurred in the largest number (6) of newly recorded river basins. None of these three species has occurred in the basins of the rivers Ipeľ, Hornád and Bodrog so far. During our study the regular occurrence of O. albicorne and Ph. montanus was examined namely in the Hornád River Basin. In the Bodrog River Basin Ph. montanus was commonly recorded.

Presence of the studied species in Slovak river subbasins during the survey period is shown on scheme (Fig. 1).

Fig. 1. Presence of Philopotamus montanus, Philopotamus ludificatus and Odontocerum albicorne in Slovak river subbasins since autumn 2003.

None of three above mentioned species was examined in the basins of the Danube, lower Váh and Latorica rivers. Despite this fact O. albicorne occurred in all three river basins, Ph. montanus was recorded in the basins of the Danube and the Latorica and Ph. ludificatus was examined in the Latorica River Basin as noted in Zaťovičová & Novikmec (2003). On the contrary, absence of O. albicorne in the Ipeľ River Basin, of Ph. ludificatus in the Morava, Ipeľ and Bodrog river basins as well as absence of all three species in the Tisa River Basin was confirmed. The other listed records (Tab. 1) confirmed the previous published data.

In the majority of cases both families were represented in reference localities regardless of altitude. Reference localities are defined by "high or good ecological status" in terms of EU Water Framework Directive (Directive 2000/60/ES), Šporka et al. (2007) and Catalogue of reference localities (Bartík et al. 2008). Other localities (10 monitoring sites with worse ecological status) inhabited by mentioned taxa were characterized mostly by higher altitude (above 400 m a.s.l.) and small or medium catchment area (10-100 km², 100-1000 km² respectively) within Carpathians ecoregion (Illies 1978, Dobiášová et al. 2006). All species are rithral representatives, Ph. ludificatus more epirithral while the others are more metarithral. It is strongly related to the hypsometric distribution and saprobic demands of studied species. Although all inhabit submountainous and mountainous regions, Ph. ludificatus was not found below 500 m a.s.l. On the contrary, the remaining species descend to uplands (200–500 m a.s.l.) as well. Regarding saprobity demands, Ph.ludificatus considered to be xenosaprobic as well as oligosaprobic indicator (Šporka (ed.) 2003) was found only in reference sites. Ph. montanus and O. albicorne tending to inhabit localities with oligosaprobic and even beta-mesosaprobic conditions occurred also in monitoring sites with moderate ecological status (Smolná - mouth, Hnilec - downstream Mníšek, Smolník - mouth) and even poor ecological status (Poprad - Veľká Lomnica). All species are considered typical rheobionts bound to the lithal substrate.

Tab. 1. List of localities with presence of Philopotamus montanus, Philopotamus ludificatus and Odontocerum albicorne.

AQEM Consortium, 2002: Manual for application of the AQEM system. A comprehensive method to access European streams using benthic invertebrates, developed for the purpose of the Water Framework Directive. Version 1.0, February 2002, 198 p.

Bartík I, Trubenová K & Haviar M, 2008: Katalóg typov povrchových vôd SR: referenčné lokality. SHMÚ&VÚVH, Bratislava, 22 p.

Directive/2000/60/EC of the European Parliament and of the Council – Establishing a framework for community action in the field of water policy. Brussels, Belgium, 23 October 2000.

Dobiášová M, Bačíková S, Scheurek K, Paľušová Z, Vančová A, Bartík I, Májovská A, Šporka F, Aroviita J, Hämäläinen H, Vehanen T, Rekolainen S, Kukkonen M, Miettinen J, Bodiš D & Slaninka I, 2006: Hodnotenie typológie útvarov povrchových vôd v SR. Priebežná správa. SHMÚ, Bratislava.

Illies J, 1978: Limnofauna Europaea. Stuttgart, Gustav Fischer Verlag.

Makovinská J, Kučárová K, Tóthová L & Haviar M (eds) 2009: Hodnotenie stavu vodných útvarov povrchových vôd Slovenska za rok 2007. Záverečná správa. VÚVH, SHMÚ, SVP, Bratislava, 70 p.

Šporka F (ed.) 2003: Vodné bezstavovce (makroevertebrata) Slovenska, súpis druhov a autekologické charakteristiky. (Slovac Aquatic Macroinvertebrates, checklist and catalogue of autecological notes). Slovenský hydrometeorologický ústav, Bratislava, 590 pp.